Intercalating Agents Like Ethidium Bromide (EtBr) Will Tend to Reduce

Intercalating agents like ethidium bromide (EtBr) will tend to reduce the twist for DNA where it binds. What other changes would accompany EtBr binding to purified plasmid DNA?

A) The linking number for the plasmid would increase.
B) The linking number for the plasmid would decrease.
C) Supercoils would become more positive (less negative) .
D) Supercoiling would become more negative (less positive) .
E) None of these changes would occur.