Which Factor Distinguishes Type I from Type II Topoisomerases

Which factor distinguishes Type I from Type II topoisomerases?

A) covalent attachment of the nucleic acid to a tyrosine
B) the presence of arginine and lysine residues in DNA binding sites
C) the use of phosphoanhydride bonds to provide energy
D) a mixture of covalent and noncovalent forces in binding of substrate
E) cleavage of the sugar-phosphate backbone in the nucleic acid substrate