A new compound isolated from mitochondria is claimed to represent a previously unrecognized carrier in the electron transfer chain. It is given the name coenzyme Z. Which line of evidence do you feel is the LEAST conclusive in assigning this compound a position in the electron transfer chain?
A) Alternate oxidation and reduction of the mitochondrion-bound coenzyme Z can be readily demonstrated.
B) Removal of coenzyme Z from the mitochondria results in a decreased rate of oxygen consumption.
C) The rate of oxidation and reduction of mitochondrion-bound coenzyme is of the same order of magnitude as the overall rate of electron transfer in mitochondria as measured by oxygen consumption.
D) The reduction potential of Z is between that of two compounds known to participate in the electron transport chain
E) When added to a mitochondrial suspension, coenzyme Z is taken up very rapidly and specifically by the mitochondria.
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