In normal mitochondria, the rate of NADH consumption (oxidation) will: A) be increased in active muscle, decreased in inactive muscle. B) be very low if the ATP synthase is inhibited, but increase when an uncoupler is added. C) decrease if mitochondrial ADP is depleted. D) decrease when cyanide is used to prevent electron transfer through the cytochrome a + a₃ complex. E) All of the answers are correct.

Question

Quizplus · Accepted Answer

All the statements accurately describe conditions affecting the rate of NADH consumption in mitochondria. A) In active muscle, the demand for ATP increases, leading to a higher rate of NADH oxidation to produce ATP. In inactive muscle, this process slows down. B) ATP synthase inhibition slows down the electron transport chain, but uncouplers increase NADH consumption by dissipating the proton gradient, thus bypassing ATP synthase. C) Depletion of ADP limits the ADP-ATP exchange necessary for oxidative phosphorylation, slowing down NADH consumption. D) Cyanide inhibits the electron transport chain by blocking cytochrome c oxidase (complex IV), reducing NADH oxidation.

In Normal Mitochondria, the Rate of NADH Consumption (Oxidation) Will