When a tissue sample is homogenized in a extractant composed of chloroform, methanol, and water, followed by addition of more water to separate the liquid phases, which molecules will remain in the methanol/water phase?

Question

Quizplus · Accepted Answer

In a chloroform-methanol-water extraction, the mixture separates into two phases: a methanol-water phase and a chloroform phase. Lipids preferentially partition into the chloroform phase due to their hydrophobic nature, while proteins and sugars, which are more hydrophilic, remain in the methanol-water phase.

When a Tissue Sample Is Homogenized in a Extractant Composed