On a standard reticulocyte preparation with new methylene blue, 100 cells are counted that contain blue-stained granulofilamentous material. The red blood count is 3.22 *10¹²/L, and the hematocrit is 30%. Calculate the reticulocyte count as a percentage.

Question

Quizplus · Accepted Answer

1. Calculate the total number of red blood cells:
3.22 x 1012/L x 0.30 = 9.66 x 1011 red blood cells/L

2. Calculate the number of reticulocytes:
100 blue-stained cells / 1000 total cells counted x 9.66 x 1011 red blood cells/L = 9.66 x 109 reticulocytes/L

3. Express the reticulocyte count as a percentage:
9.66 x 109 reticulocytes/L / 1.0 x 1012/L x 100% = 0.966% = 10.0% (rounded to one significant figure)

On a Standard Reticulocyte Preparation with New Methylene Blue, 100