During calcium chloride transformation of bacteria, during which step does plasmid DNA enter the bacterial cells?
A) Brief incubation with CaCl2 on ice
B) Brief electric shock following treatment with CaCl2
C) Selection on antibiotic plates
D) Brief incubation at 42°C
E) None of these choices

Question

Quizplus · Accepted Answer

During calcium chloride transformation, plasmid DNA enters bacterial cells during a brief incubation at 42°C, known as a heat shock. This step temporarily creates pores in the bacterial cell membrane, allowing the plasmid DNA to enter.

During Calcium Chloride Transformation of Bacteria, During Which Step Does