Which of the following is an INCORRECT statement about restriction enzymes?
A) Most restriction enzymes are isolated from bacteria.
B) Restriction enzymes usually recognize palindromic sequences.
C) Restriction enzymes create phosphodiester bonds between pieces of DNA in a cloning experiment.
D) Restriction enzymes can cut to create overlapping single-stranded ends of DNA.
E) Restriction enzymes can cut to create blunt-ended pieces of DNA.

Question

Quizplus · Accepted Answer

Restriction enzymes cleave DNA by hydrolyzing phosphodiester bonds. However, in a cloning experiment, ligases are used to create phosphodiester bonds between pieces of DNA.

Which of the Following Is an INCORRECT Statement About Restriction