During molecular cloning, a gene of interest (insulin) is inserted into a bacterial structure called a ________ and enters a bacterial cell through a process called ________.
A) chromosome; electrophoresis
B) nucleus; transformation
C) plasmid; transcription
D) plasmid; transformation
E) genome; transformation

Question

Quizplus · Accepted Answer

During molecular cloning, a gene of interest is typically inserted into a plasmid, which is a small, circular piece of DNA that can replicate independently within a bacterial cell. The plasmid is then introduced into the bacterial cell through a process called transformation, which involves the uptake of foreign DNA by the cell. Therefore, the correct answer is D, plasmid and transformation.

During Molecular Cloning, a Gene of Interest (Insulin) Is Inserted