Which of the following is true of trypsin?
A) It does not affect any peptide bonds.
B) It hydrolyzes all peptide bonds.
C) It hydrolyzes peptide bonds to leucine and phenylalanine.
D) It hydrolyzes peptide bonds to arginine and lysine.

Question

Quizplus · Accepted Answer

Trypsin is a proteolytic enzyme that cleaves peptide bonds specifically at the carboxyl side of arginine and lysine residues. This selectivity is due to the presence of specific amino acid residues in the active site of trypsin that interact with the side chains of arginine and lysine. Trypsin does not hydrolyze all peptide bonds or affect any peptide bonds. Moreover, trypsin has a preference for hydrophobic amino acids such as leucine and phenylalanine at the carboxyl side of the cleavage site.

Which of the Following Is True of Trypsin