What biological technique has provided evidence used in establishing the eukaryotic superkingdoms?

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Molecular sequence comparisons, such as comparing the DNA or RNA sequences of different organisms, can provide insight into their evolutionary relationships and help establish superkingdoms. This technique has helped identify three main superkingdoms of eukaryotes: Opisthokonta, Archaeplastida, and Unikonta. Bacterial culturing, the identification of land plants, and visualizing cell shape may provide information about specific groups of organisms, but are not as widely applicable in determining superkingdoms.

What Biological Technique Has Provided Evidence Used in Establishing the Eukaryotic