In the diagram below, the horizontal lines represent DNA strands in a double-stranded molecule, the vertical lines mark the positions of cleavage sites for a particular restriction enzyme, the arrows show the positions of primers used in the polymerase chain reaction (PCR) to amplify the region, and the numbers are the number of nucleotides between successive restriction sites. If this particular DNA molecule is amplified and then cleaved with the restriction enzymes, what size band(s) would be observed in a gel?
A)5 kb
B)5 kb and 3 kb
C)5 kb, 3 kb, and 2 kb
D)3 kb and 2 kb
E)None of the answer options is correct.

Question

In the diagram below, the horizontal lines represent DNA strands in a double-stranded molecule, the vertical lines mark the positions of cleavage sites for a particular restriction enzyme, the arrows show the positions of primers used in the polymerase chain reaction (PCR) to amplify the region, and the numbers are the number of nucleotides between successive restriction sites. If this particular DNA molecule is amplified and then cleaved with the restriction enzymes, what size band(s) would be observed in a gel? 
A)5 kb 
B)5 kb and 3 kb 
C)5 kb, 3 kb, and 2 kb 
D)3 kb and 2 kb 
E)None of the answer options is correct.

Quizplus · Accepted Answer

The Answer of In the diagram below, the horizontal lines represent DNA strands in a double-stranded molecule, the vertical lines mark the positions of cleavage sites for a particular restriction enzyme, the arrows show the positions of primers used in the polymerase chain reaction (PCR) to amplify the region, and the numbers are the number of nucleotides between successive restriction sites. If this particular DNA molecule is amplified and then cleaved with the restriction enzymes, what size band(s) would be observed in a gel? 
A)5 kb 
B)5 kb and 3 kb 
C)5 kb, 3 kb, and 2 kb 
D)3 kb and 2 kb 
E)None of the answer options is correct.

In the Diagram Below, the Horizontal Lines Represent DNA Strands