Once the histones are removed from a metaphase chromosome, one can see DNA spreading out in loops around the supporting protein structure, called the:

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Once the histones are removed, the chromosome is supported by a protein structure called the scaffold, which DNA loops around. This is the most appropriate choice for the answer. The 1400-nm fiber, 300-nm fiber, and 2-nm fiber are all levels of chromatin packaging that occur before the removal of histones. The beads on a string refer to the appearance of chromatin at the level of nucleosomes, which are made up of DNA wrapped around histone proteins.

Once the Histones Are Removed from a Metaphase Chromosome, One