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A Student in a Biology Laboratory Designs Oligonucleotide Primers to Carry

Question 101

Essay

A student in a biology laboratory designs oligonucleotide primers to carry out the polymerase chain reaction. However when ordering the sequences, she reverses the 3' and 5' ends. The vendor synthesizes the sequences as ordered and returns them to the student. Will they produce an amplified product? Please explain your answer.

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They will not work. In fact, they will n...

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