Which of the following statements is/are correct for glucose testing by reagent strip?
I. Glucose oxidase reacts with glucose in the urine.
II. Non-glucose–reducing substances fail to react.
III. Ascorbic acid may cause a false-negative reaction.
IV. Hydrogen peroxide and peroxidase react to oxidize a chromogen.

Question

Quizplus · Accepted Answer

All statements are correct regarding glucose testing by reagent strip. Glucose oxidase reacts with glucose in the urine to produce gluconic acid and hydrogen peroxide. Non-glucose–reducing substances do not react, making the test specific for glucose. Ascorbic acid can consume the hydrogen peroxide, leading to a false-negative reaction. Finally, hydrogen peroxide and peroxidase react to oxidize a chromogen, producing a color change that indicates the presence of glucose.

Which of the Following Statements Is/are Correct for Glucose Testing