Dilution of CSF prior to performing a total cell count should be made using:

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Dilution of CSF prior to performing a total cell count should be made using normal saline as it will provide an isotonic solution and preserve the cell morphology. Deionized water can cause cell lysis and alter the cell counts, while acetic acid and acetic acid containing methylene blue are used for differential cell counts, not total cell counts.

Dilution of CSF Prior to Performing a Total Cell Count