Microscopic examination of stools mixed with Sudan III stain,glacial acetic acid,and then heated will show small orange-red droplets that represent:
A) Soaps
B) Fatty acids and soaps
C) Fatty acids and neutral fats
D) Fatty acids, soaps, and neutral fats

Question

Quizplus · Accepted Answer

Sudan III stain is used to detect lipids in the stool. Glacial acetic acid is used to dissolve excess mucus and debris that may obscure the lipids. On heating, the neutral fats and fatty acids will dissolve and combine with the sudan III. Therefore, the small orange-red droplets seen under microscopic examination represent the presence of fatty acids, soaps, and neutral fats in the stool.

Microscopic Examination of Stools Mixed with Sudan III Stain,glacial Acetic