Question 1

Immersion oil increases the amount of light passing through a specimen and entering the objective lens.

Accepted Answer

Immersion oil has a higher refractive index than air and helps to reduce the loss of light due to refraction and reflection. It also helps to increase resolution and clarity by reducing spherical aberration.

Question 2

A 45$\times$ objective and a 10$\times$ ocular produce a total magnification of

Accepted Answer

To calculate the total magnification, we need to multiply the magnification of the objective lens by the magnification of the ocular lens.

Magnification of objective lens = 45
Magnification of ocular lens = 10

Total magnification = Magnification of objective lens x Magnification of ocular lens  
= 45 x 10 
= 450

Therefore, the total magnification is 450x.

Question 3

If the objective lenses of a microscope can be changed without losing focus on the specimen, they are said to be

Accepted Answer

If the objective lenses of a microscope are parfocal, then they can be changed without the need to refocus on the specimen. This is a desirable feature, as it saves time and helps to prevent damage to the specimen. Equifocal and totifocal are not commonly used terms in microscopy, and optifocal is not a recognized term at all.

Question 4

Light rays are refracted (bent) when they cross the interface between materials with different refractive indices.

Accepted Answer

This statement is true.

Question 5

A microscope that exposes specimens to ultraviolet, violet, or blue light and forms an image with the light emitted at a different wavelength is called a __________ microscope.

Accepted Answer

A microscopy technique that uses a fluorescence microscope is called a fluorescence microscope. This microscope uses high-intensity light of a specific wavelength to excite fluorescent molecules in a sample, causing them to emit light at a longer wavelength, creating an image. Therefore, fluorescence microscopy is used to study biological molecules, cells, and tissues.

Question 6

The __________ is the point at which a lens focuses parallel beams of light.

Accepted Answer

The answer of The __________ is the point at which...

Question 7

Resolution decreases when the wavelength of the illuminating light decreases.

Accepted Answer

The answer of Resolution decreases when the wavelength of the...

Question 8

A light microscope with an objective lens numerical aperture of 0.65 is capable of allowing two objects 400 nm apart to be distinguished when using light with a wavelength of 420 nm.
Section 2.02

Accepted Answer

This is true according to the resolution equation, which states that resolution is equal to 0.61 times the wavelength of light divided by the numerical aperture of the objective lens. Plugging in the values given, we get a resolution of approximately 407 nm, which is smaller than the distance of 400 nm between the objects. Therefore, they can be distinguished.

Question 9

Immersion oil can be used to increase the resolution achieved with some microscope lenses because it increases the __________ between the specimen and the objective lens.

Accepted Answer

Immersion oil has the same refractive index as glass, which reduces the loss of light due to refraction and increases the numerical aperture of the microscope. This improvement in numerical aperture allows for a higher resolution and better clarity of the specimen. Immersion oil does not directly affect optical density.

Question 10

Confocal microscopes exhibit improved contrast and resolution by

Accepted Answer

Blocking out stray light with an aperture located above the objective lens helps to eliminate background interference and improve contrast and resolution in confocal microscopy.

Question 11

The useful magnification of a light microscope is limited by the ___________ of the light source being utilized.
Section 2.02

Accepted Answer

The answer of The useful magnification of a light microscope...

Question 12

The __________ is the distance between the specimen and the objective lens when the specimen is in focus.

Accepted Answer

The answer of The __________ is the distance between the...

Question 13

A 30$\times$ objective and a 20$\times$ ocular produce a total magnification of

Accepted Answer

Total magnification is calculated by multiplying the magnification of the objective and the ocular. Therefore, the total magnification is 30 x 20 = 600. The choices A, B, and C are incorrect because they either do not include the multiplication or have incorrect values for the magnification of the objective and ocular.

Question 14

The special dyes used in fluorescence microscopy that absorb light at one wavelength and emit light at a different wavelength are called __________.

Accepted Answer

The answer of The special dyes used in fluorescence microscopy...

Question 15

It is possible to build a light microscope capable of 10,000$\times$ magnification, but the image would not be sharp because resolution is independent of magnification.

Accepted Answer

Magnification increases the size of the image, but resolution, which is the ability to distinguish two points as separate, depends on the wavelength of light and the numerical aperture of the microscope. High magnification without matching high resolution will result in a larger but blurry image.

Question 16

The __________ is the distance between the center of a lens and the point at which it focuses parallel beams of light.

Accepted Answer

The answer of The __________ is the distance between the...

Question 17

Confocal microscopes, in combination with specialized computer software, can be used to create three-dimensional images of cell structures.

Accepted Answer

Confocal microscopes use lasers and specialized optical systems to produce high-resolution images of cellular structures, and the images can be processed with computer software to create 3D reconstructions.

Question 18

Immersion oil is used to prevent a specimen from drying out.

Accepted Answer

Immersion oil is used to increase the resolution of a microscope by reducing the refraction of light between the slide and the objective lens, not to prevent a specimen from drying out.

Question 19

A substage condenser is used to focus light onto the specimen, which increases the resolution of a light microscope.

Accepted Answer

A substage condenser is an essential component of a light microscope that ensures the concentration and focusing of light onto the specimen. This increased light intensity enhances the resolution of the microscope, allowing for more detailed visualization of the specimen under examination.

Question 20

Which of these microscopes can be used to create high-resolution three-dimensional images of cells?

Accepted Answer

Confocal microscopy uses a laser to create precise optical sections of the sample, which can be stacked together to create a high-resolution 3D image. This is particularly useful for studying cells and tissues in detail. Differential interference contrast, dark field, and phase-contrast microscopy are all useful for visualizing different aspects of cells, but they do not create 3D images with the same level of detail as confocal microscopy.

Quiz 2: The Study of Microbial Structure: Microscopy and Specimen Preparation

Immersion oil increases the amount of light passing through a specimen and entering the objective lens.

A 45 $\times$ objective and a 10 $\times$ ocular produce a total magnification of

If the objective lenses of a microscope can be changed without losing focus on the specimen, they are said to be

Light rays are refracted (bent) when they cross the interface between materials with different refractive indices.

A microscope that exposes specimens to ultraviolet, violet, or blue light and forms an image with the light emitted at a different wavelength is called a __________ microscope.

The __________ is the point at which a lens focuses parallel beams of light.

Resolution decreases when the wavelength of the illuminating light decreases.

A light microscope with an objective lens numerical aperture of 0.65 is capable of allowing two objects 400 nm apart to be distinguished when using light with a wavelength of 420 nm. Section 2.02

Immersion oil can be used to increase the resolution achieved with some microscope lenses because it increases the __________ between the specimen and the objective lens.

Confocal microscopes exhibit improved contrast and resolution by

The useful magnification of a light microscope is limited by the ___________ of the light source being utilized. Section 2.02

The __________ is the distance between the specimen and the objective lens when the specimen is in focus.

A 30 $\times$ objective and a 20 $\times$ ocular produce a total magnification of

The special dyes used in fluorescence microscopy that absorb light at one wavelength and emit light at a different wavelength are called __________.

It is possible to build a light microscope capable of 10,000 $\times$ magnification, but the image would not be sharp because resolution is independent of magnification.

The __________ is the distance between the center of a lens and the point at which it focuses parallel beams of light.

Confocal microscopes, in combination with specialized computer software, can be used to create three-dimensional images of cell structures.

Immersion oil is used to prevent a specimen from drying out.

A substage condenser is used to focus light onto the specimen, which increases the resolution of a light microscope.

Which of these microscopes can be used to create high-resolution three-dimensional images of cells?

Quiz 2: The Study of Microbial Structure: Microscopy and Specimen Preparation

Immersion oil increases the amount of light passing through a specimen and entering the objective lens.

A 45 ×\times× objective and a 10 ×\times× ocular produce a total magnification of

If the objective lenses of a microscope can be changed without losing focus on the specimen, they are said to be

Light rays are refracted (bent) when they cross the interface between materials with different refractive indices.

A microscope that exposes specimens to ultraviolet, violet, or blue light and forms an image with the light emitted at a different wavelength is called a __________ microscope.

The __________ is the point at which a lens focuses parallel beams of light.

Resolution decreases when the wavelength of the illuminating light decreases.

A light microscope with an objective lens numerical aperture of 0.65 is capable of allowing two objects 400 nm apart to be distinguished when using light with a wavelength of 420 nm. Section 2.02

Immersion oil can be used to increase the resolution achieved with some microscope lenses because it increases the __________ between the specimen and the objective lens.

Confocal microscopes exhibit improved contrast and resolution by

The useful magnification of a light microscope is limited by the ___________ of the light source being utilized. Section 2.02

The __________ is the distance between the specimen and the objective lens when the specimen is in focus.

A 30 ×\times× objective and a 20 ×\times× ocular produce a total magnification of

The special dyes used in fluorescence microscopy that absorb light at one wavelength and emit light at a different wavelength are called __________.

It is possible to build a light microscope capable of 10,000 ×\times× magnification, but the image would not be sharp because resolution is independent of magnification.

The __________ is the distance between the center of a lens and the point at which it focuses parallel beams of light.

Confocal microscopes, in combination with specialized computer software, can be used to create three-dimensional images of cell structures.

Immersion oil is used to prevent a specimen from drying out.

A substage condenser is used to focus light onto the specimen, which increases the resolution of a light microscope.

Which of these microscopes can be used to create high-resolution three-dimensional images of cells?

A 45 $\times$ objective and a 10 $\times$ ocular produce a total magnification of

A 30 $\times$ objective and a 20 $\times$ ocular produce a total magnification of

It is possible to build a light microscope capable of 10,000 $\times$ magnification, but the image would not be sharp because resolution is independent of magnification.