Question 1

Which of the following is a reported parameter for the ADVIA 120 but not for the LH 750 instrument?

Accepted Answer

CHCM (Cell Hemoglobin Concentration Mean) is a parameter reported by the ADVIA 120 hematology analyzer but not by the LH 750.

Question 2

Which of the following analyzers measures reticulocytes by sphering the blood cells prior to analysis?

Accepted Answer

B

Question 3

Which of the following analyzers uses radio frequency to detect and differentiate leukocytes?

Accepted Answer

The Sysmex XE-2100 is the only analyzer among the options given that uses radio frequency to detect and differentiate leukocytes. The Bayer Advia 2120 uses impedance technology, the Abbott Sapphire uses laser light scatter technology, and the Coulter Gen-S uses impedance and light scatter technology.

Question 4

Hydrodynamic focusing is used in automated cell-counting instruments to:

Accepted Answer

Hydrodynamic focusing is a technique used in flow cytometry and automated cell-counting instruments to align cells so that they pass through the detection aperture one at a time. This prevents multiple cells from passing through the aperture simultaneously, which could lead to inaccurate counts and analyses. This process is known as preventing coincidence passage.

Question 5

What cellular characteristic is evaluated by radio frequency?

Accepted Answer

The answer of What cellular characteristic is evaluated by radio...

Question 6

Which analyzer has the ability to quantify nucleated RBCs and calculate a corrected WBC count?

Accepted Answer

The answer of Which analyzer has the ability to quantify...

Question 7

Cell volume and cell number are required elements for which of the following?

Accepted Answer

RBC histograms require information on cell volume and cell number to plot the distribution of red blood cells by their size, which is crucial for identifying conditions like anemia or polycythemia.

Question 8

Which analyzer does not use cyanide-free detection of hemoglobin?

Accepted Answer

The Advia 120 analyzer uses cyanide-based detection methods for hemoglobin, while the other options on the list use cyanide-free methods.

Question 9

The Sysmex XE-2100 instrument printout reports a middle fluorescent ratio of 7.0 and a high fluorescent ratio of 1.3. What is the immature reticulocyte fraction?

Accepted Answer

The immature reticulocyte fraction (IRF) can be calculated using the middle fluorescent ratio (MFR) and the high fluorescent ratio (HFR) provided by the Sysmex XE-2100 instrument. The specific formula or method to derive the IRF from the MFR and HFR values is not provided in the question, but based on the given options and the typical range for IRF values, option C (8.3) is the most plausible answer. IRF values are usually expressed as a percentage, indicating the proportion of immature reticulocytes among total reticulocytes, and an IRF of 8.3% falls within a realistic range for this parameter.

Question 10

Which parameter does the Sysmex XE-2100 instrument determine by using light-scattering and fluorescence intensity characteristics?

Accepted Answer

The answer of Which parameter does the Sysmex XE-2100 instrument...

Question 11

Which of the following instruments does not rely on impedance to enumerate cells?

Accepted Answer

The answer of Which of the following instruments does not...

Question 12

Which of the following represents the degree of light scatter that reflects cell complexity on the CELL-DYN Sapphire?

Accepted Answer

The CELL-DYN Sapphire measures light scatter at 7 degrees, which reflects cell complexity.

Question 13

Which parameter does the Coulter LH 750 instrument directly measure from a histogram?

Accepted Answer

The Coulter LH 750 instrument directly measures the platelet count from the histogram.

Question 14

In the reticulocyte determination, using the Sysmex XE-2100, the fluorescent dye containing oxazine and polymethine penetrates the cell membranes and stains the blood cells. What does the oxazine bind to within the cells?

Accepted Answer

The oxazine dye used in the Sysmex XE-2100 binds to residual RNA within the cells, allowing for the identification and quantification of reticulocytes.

Question 15

The principle that relies on detecting resistance as cells pass through a photo-detector and is used by most of the major hematology analyzers is:

Accepted Answer

The principle that relies on detecting resistance as cells pass through a photo-detector is called impedance, and it is the most commonly used technology in modern hematology analyzers. Spectrophotometry involves analyzing the absorption or transmission of light by a substance, and it is not typically used for cell counting or differentiation. Radio frequency and MAPSS technology are not commonly used in hematology analyzers.

Question 16

Which of the following parameters are calculated on the Coulter LH?

Accepted Answer

The answer of Which of the following parameters are calculated...

Question 17

Regarding the CELL-DYN Sapphire, which parameter is determined by the impedance principle?

Accepted Answer

The impedance principle is used to count and size cells in a hematology analyzer. Platelets are also counted using the impedance principle. Therefore, the correct answer is C for Platelet count.

Question 18

Which analyzer measures platelets in the same channel as reticulocytes?

Accepted Answer

The Sysmex XE-2100 measures platelets in the same channel as reticulocytes.

Question 19

Which instrument does not use fluorescence intensity in its determination of reticulocytes?

Accepted Answer

The LH 750 does not use fluorescence intensity in its determination of reticulocytes. The other instruments listed do use fluorescence intensity to determine reticulocyte counts.

Question 20

Which of the following binds to residual RNA in reticulocytes on the Sysmex XE-2100 analyzer?

Accepted Answer

The answer of Which of the following binds to residual...

Quiz 39: Automation in Hematology