A mutation results in an amino acid substitution at a site distant from the active site of an enzyme.How might this amino acid change alter the active site and substrate specificity of the enzyme?
A)by changing the optimum temperature for the enzyme
B)by changing the intracellular location of the enzyme
C)by changing the binding site for an allosteric regulator
D)by changing the conformation of the enzyme

Question

Quizplus · Accepted Answer

Amino acid substitutions can lead to changes in the enzyme's conformation, which can ultimately impact the active site and the enzyme's ability to interact with substrate(s). It is possible that this change could alter the substrate specificity, making the enzyme less or more effective at catalyzing the reaction of interest. Options A, B, and C are less likely to have a direct impact on the active site and substrate specificity of the enzyme.

A Mutation Results in an Amino Acid Substitution at a Site