Question 1

Gene cloning requires placing the gene of interest into a cloning vector.A good cloning vector must have which of the following?

Accepted Answer

all of the above

Question 2

Which of the following is NOT true regarding DNA gel electrophoresis?

Accepted Answer

Larger DNA fragments will migrate farther than smaller DNA fragments will .

Question 3

You are studying a human gene that requires posttranslational modification.What featuresis important in picking your vector?

Accepted Answer

It needs to be an expression vector .

Question 4

Recombinant DNA technology has led to advances in which of the following?

Accepted Answer

all of the above

Question 5

Which of the following is an advantage of whole-genome shotgun sequencing compared to map-based sequencing?

Accepted Answer

The shotgun approa ch is highly auto mated.

Question 6

A person has been arrested and charged for murder.DNA fingerprint analyses were performed on DNA found at the crime scene left laneand on DNA from the suspect right lane).Does this evidence place the person at the scene of the crime?

Accepted Answer

This evidence does not prove that the person was at the crime scene , though the person still could have been there .

Question 7

Thomas Hunt Morgan identified the white-eyed mutant using Drosophila and later determined it to be a sex-linked gene.This is an example of

Accepted Answer

forward genetics .

Question 8

Gene therapy involves transferring desired genes into diseased cells.Typically this is performed only on nonreproductive cells somatic cellsbecause there are ethical concerns in using germ-line gene therapy.Why?

Accepted Answer

This could alter the gene pool.

Question 9

Which of the following is NOT true regarding the field of bioinformatics?

Accepted Answer

High subscription costs for access to bioinformatics databases make them impractical for students and many researchers.

Question 10

Molecular genetics is done with which class of restriction enzymes?

Accepted Answer

c lass III

Question 11

Which of the following is a product that has resulted or improved because of recombinant DNA technology?

Accepted Answer

all of the above

Question 12

Which of the following is NOT true regarding the human genome project?

Accepted Answer

Because of differences among the many gr oups working on the project , it finishe d five years behind schedule.

Question 13

To clone a large piece of DNA,which of the following bacterial cloning vectors should be used?

Accepted Answer

b acterial artificial chromosome

Question 14

Which of the following is NOT a required step in genetically engineering bacteria that can produce a human protein?

Accepted Answer

Insert the human protein into bacteria .

Question 15

A DNA probe with a sequence similar to that of the gene of interest is often used to screen a DNA library.What could you use as a probe if the sequence of the gene of interest is not known?

Accepted Answer

e ither A or B

Question 16

DNA libraries are constructed and screened for genes of interest.If you were interested in identifying genes expressed in cancer cells,why might you choose to use a cDNA library instead of a genomic DNA library?

Accepted Answer

A cDNA library is enriched for genes that are actively transcribed in your cell of interest.

Question 17

During dideoxy sequencing reactions,dideoxyribonucleoside triphosphates ddNTPsare added in small amounts to terminate the sequencing reaction.How do ddNTPs stop a sequencing reaction?

Accepted Answer

ddNTPs lack a hydroxyl - OH )
 group at their 3 ′ end .

Question 18

The human genome contains three genes for enolase $\alpha$,$\beta$,$\gamma$),whereas yeast contains just one gene for enolase.The three human enolase genes are __________ of each other,whereas the human and yeast enolase genes are __________ of each other.

Accepted Answer

othologs; paralogs

Question 19

A restriction enzyme that has a long recognition sequence will cut a genome ________ times compared to a restriction enzyme with a shorter recognition sequence.

Accepted Answer

fewer

Question 20

Which is NOT true of a genetic map?

Accepted Answer

Genetic maps are not limited to single-locus traits; they can be constructed using multiple loci and are based on the frequency of recombination between markers.

Quiz 14: Molecular Genetic Analysis, Biotechnology, and Genomics

Gene cloning requires placing the gene of interest into a cloning vector.A good cloning vector must have which of the following?

Which of the following is NOT true regarding DNA gel electrophoresis?

You are studying a human gene that requires posttranslational modification.What featuresis important in picking your vector?

Recombinant DNA technology has led to advances in which of the following?

Which of the following is an advantage of whole-genome shotgun sequencing compared to map-based sequencing?

A person has been arrested and charged for murder.DNA fingerprint analyses were performed on DNA found at the crime scene left laneand on DNA from the suspect right lane) .Does this evidence place the person at the scene of the crime?

Thomas Hunt Morgan identified the white-eyed mutant using Drosophila and later determined it to be a sex-linked gene.This is an example of

Gene therapy involves transferring desired genes into diseased cells.Typically this is performed only on nonreproductive cells somatic cellsbecause there are ethical concerns in using germ-line gene therapy.Why?

Which of the following is NOT true regarding the field of bioinformatics?

Molecular genetics is done with which class of restriction enzymes?

Which of the following is a product that has resulted or improved because of recombinant DNA technology?

Which of the following is NOT true regarding the human genome project?

To clone a large piece of DNA,which of the following bacterial cloning vectors should be used?

Which of the following is NOT a required step in genetically engineering bacteria that can produce a human protein?

A DNA probe with a sequence similar to that of the gene of interest is often used to screen a DNA library.What could you use as a probe if the sequence of the gene of interest is not known?

DNA libraries are constructed and screened for genes of interest.If you were interested in identifying genes expressed in cancer cells,why might you choose to use a cDNA library instead of a genomic DNA library?

During dideoxy sequencing reactions,dideoxyribonucleoside triphosphates ddNTPsare added in small amounts to terminate the sequencing reaction.How do ddNTPs stop a sequencing reaction?

The human genome contains three genes for enolase $\alpha$ , $\beta$ , $\gamma$ ) ,whereas yeast contains just one gene for enolase.The three human enolase genes are of each other,whereas the human and yeast enolase genes are of each other.

A restriction enzyme that has a long recognition sequence will cut a genome ________ times compared to a restriction enzyme with a shorter recognition sequence.

Which is NOT true of a genetic map?

Quiz 14: Molecular Genetic Analysis, Biotechnology, and Genomics

Gene cloning requires placing the gene of interest into a cloning vector.A good cloning vector must have which of the following?

Which of the following is NOT true regarding DNA gel electrophoresis?

You are studying a human gene that requires posttranslational modification.What featuresis important in picking your vector?

Recombinant DNA technology has led to advances in which of the following?

Which of the following is an advantage of whole-genome shotgun sequencing compared to map-based sequencing?

A person has been arrested and charged for murder.DNA fingerprint analyses were performed on DNA found at the crime scene left laneand on DNA from the suspect right lane) .Does this evidence place the person at the scene of the crime?

Thomas Hunt Morgan identified the white-eyed mutant using Drosophila and later determined it to be a sex-linked gene.This is an example of

Gene therapy involves transferring desired genes into diseased cells.Typically this is performed only on nonreproductive cells somatic cellsbecause there are ethical concerns in using germ-line gene therapy.Why?

Which of the following is NOT true regarding the field of bioinformatics?

Molecular genetics is done with which class of restriction enzymes?

Which of the following is a product that has resulted or improved because of recombinant DNA technology?

Which of the following is NOT true regarding the human genome project?

To clone a large piece of DNA,which of the following bacterial cloning vectors should be used?

Which of the following is NOT a required step in genetically engineering bacteria that can produce a human protein?

A DNA probe with a sequence similar to that of the gene of interest is often used to screen a DNA library.What could you use as a probe if the sequence of the gene of interest is not known?

DNA libraries are constructed and screened for genes of interest.If you were interested in identifying genes expressed in cancer cells,why might you choose to use a cDNA library instead of a genomic DNA library?

During dideoxy sequencing reactions,dideoxyribonucleoside triphosphates ddNTPsare added in small amounts to terminate the sequencing reaction.How do ddNTPs stop a sequencing reaction?

The human genome contains three genes for enolase α\alphaα , β\betaβ , γ\gammaγ ) ,whereas yeast contains just one gene for enolase.The three human enolase genes are __________ of each other,whereas the human and yeast enolase genes are __________ of each other.

A restriction enzyme that has a long recognition sequence will cut a genome ________ times compared to a restriction enzyme with a shorter recognition sequence.

Which is NOT true of a genetic map?

The human genome contains three genes for enolase $\alpha$ , $\beta$ , $\gamma$ ) ,whereas yeast contains just one gene for enolase.The three human enolase genes are of each other,whereas the human and yeast enolase genes are of each other.