Question 1

The clinical microbiology laboratory just received a sputum (deep-lung) sample that so far has been very slow-growing in nonselective agar, as well as not Gram stainable. What tests should be performed next, and why?

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Question 2

Compare and contrast the use of the serum antibody ELISA with the antigen-capture ELISA in the detection of Ebola.

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Question 3

Compare and contrast the handling of rabies (a category III organism) with Lassa fever (a category IV organism).

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Question 4

As a disease detective investigating an outbreak in the cafeteria of your college, what would be some of the first steps you would need to take to begin your investigation, other than laboratory testing?

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Question 5

Compare and contrast the use of point-of-care rapid tests as diagnostic methods in doctors' offices.

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Question 6

How were Koch's postulates modified in the discovery of Tropheryma whipplei, the causative agent of Whipple's disease?

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Question 7

Compare and contrast the epidemiological terms "endemic," "epidemic," and "pandemic." Give an example of each to support your answer.

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Question 8

How does a multiplex PCR reaction differ from a traditional PCR detection method? In addition, describe an example of how multiplex can be used in diagnostics.

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Question 9

How would a serum antibody ELISA work in the detection of Lyme disease?

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Question 10

How have changes within our human culture caused new diseases to emerge? Explain your answer using a specific example that supports your statements.

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Question 11

Why are clinical viral diseases more challenging to diagnose than are clinical bacterial diseases?

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Question 12

Describe what the One Health Initiative is and the role it played in the 2006 outbreak of food-borne O157:H7 in spinach.

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Question 13

How can reverse transcriptase PCR (RT-PCR) be used to determine the presence and viral load of HIV infection?

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Question 14

Describe the chain of command that is followed when a patient sees his or her doctor and is diagnosed with a reportable disease such as listeriosis.

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Question 15

Once cultured, bacteria from septic infections or urine can be quickly identified by MALDI-TOF-MS. What is this technique and how does it work?

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Quiz 28: Clinical Microbiology and Epidemiology