Which of the following experimental approaches would be useful for studying protein-protein interactions within a cell?
A) Differential interference-contrast microscopy
B) Differential centrifugation
C) Fluorescence pulse field gel electrophoresis (FPFGE)
D) Fluorescence resonance energy transfer (FRET)

Question

Quizplus · Accepted Answer

Fluorescence resonance energy transfer (FRET) is a useful experimental approach for studying protein-protein interactions within a cell. FRET involves labeling two proteins of interest with different fluorescent dyes and measuring the transfer of energy from one dye to the other as the proteins interact. This allows for the detection and measurement of protein-protein interactions within living cells. Differential interference-contrast microscopy (A) and differential centrifugation (B) are not useful for directly studying protein-protein interactions, as they are primarily used for separating cellular components based on size or density. Fluorescence pulse field gel electrophoresis (FPFGE) (C) is a technique for separating and visualizing large DNA fragments based on their size, and is not commonly used for studying protein-protein interactions.

Which of the Following Experimental Approaches Would Be Useful for Studying