Specific proteins can be localized in the transmission electron microscope by use of
A) positive staining.
B) negative staining.
C) fluorescent-labeled antibodies.
D) gold-labeled antibodies.

Question

Quizplus · Accepted Answer

Gold-labeled antibodies can be used for specific protein localization in the transmission electron microscope. The gold particles bind to the target protein and can be seen in the microscope, providing a highly specific and sensitive method of localization. Positive staining can also be used to visualize proteins, but it is less specific and may not accurately reflect protein localization. Fluorescent-labeled antibodies are more commonly used for localization in the light microscope. Negative staining is used primarily to visualize the surface features of particles or cells.

Specific Proteins Can Be Localized in the Transmission Electron Microscope