Experiments in Drosophila found that XXYflies were female and XO flies were male. This demonstrated that the number of Y chromosomes was critical in determining sex.

Name: Experiments in Drosophila found that XXY flies were female and XO flies were male. This demonstrated that the number of Y chromosomes was critical in determining sex.
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In Drosophila, two X chromosomes results in female. One X chromosome results in male, even in the absence of a Y chromosome.

Experiments in Drosophila Found That XXY flies Were Female and XO

Experiments in Drosophila Found That XXYflies Were Female and XO