Question 1

DNA fragments separated on an agarose gel can be visualized after staining with ____

Accepted Answer

Ethidium bromide is a commonly used stain for visualizing DNA fragments on an agarose gel under UV light. It intercalates between the DNA bases, allowing the fragments to be seen.

Question 2

A gene whose expression helps to identify transformed cells is known as ____

Accepted Answer

Selectable markers help in identifying or selecting the cells that have been successfully transformed with a new gene.

Question 3

A single strand of nucleic acid tagged with a radioactive molecule is called

Accepted Answer

A probe is a single-stranded sequence of DNA or RNA used in molecular biology to detect the presence of complementary sequences by hybridization. Probes are often tagged with radioactive molecules to allow for their detection in various assays.

Question 4

There is a restriction endonuclease called Eco RI. What does 'co' part in it stand for?

Accepted Answer

The 'co' in Eco RI stands for 'coli', as in Escherichia coli, the bacterium from which this restriction enzyme was first isolated.

Question 5

Agarose extracted from sea weeds finds use in ____

Accepted Answer

Agarose, extracted from seaweed, is primarily used in gel electrophoresis for the separation of nucleic acids (DNA or RNA) based on their size. This makes it a fundamental tool in molecular biology for analyzing and purifying nucleic acids.

Question 6

Animal biotechnology involves

Accepted Answer

Animal biotechnology encompasses the use of recombinant DNA technology to produce valuable products in animals, the rapid multiplication of animals with desired genotypes, and the alteration of genes to enhance desirable traits.

Question 7

Animal cell cultures are used widely for the production of

Accepted Answer

Monoclonal antibodies (mabs) are produced using animal cell cultures, as they require complex post-translational modifications that can only be performed by eukaryotic cells.

Question 8

The first vaccine developed from animal cell culture was

Accepted Answer

The first vaccine developed from animal cell culture was the Polio vaccine. This method allowed for the large-scale production of vaccines and was a significant advancement in vaccine technology.

Question 9

Which of the following are commonly produced in animal cell cultures

Accepted Answer

Animal cell cultures are widely used for the production of interferons, monoclonal antibodies (mAb), and vaccines, making all of these options correct.

Question 10

The cell line used for the production of polio vaccine was

Accepted Answer

The polio vaccine was historically produced using primary cultures from primate kidney cells. These cells were used because they could support the growth of the poliovirus, allowing for the production of vaccine doses.

Question 11

Recombinant proteins are

Accepted Answer

Recombinant proteins are produced by inserting a gene encoding the desired protein into a host cell using recombinant DNA (rDNA) technology, allowing the host cell to express the protein.

Question 12

Interferons are

Accepted Answer

Interferons are proteins that are part of the immune response and are primarily known for their role in inhibiting viral replication within host cells.

Question 13

The virus commonly used to infect cell cultures for the production of interferon is

Accepted Answer

Sendai virus is commonly used to infect cell cultures for the production of interferon because it is highly efficient at inducing the interferon response in infected cells.

Question 14

Hybrid antibodies are

Accepted Answer

The answer of Hybrid antibodies are
...

Question 15

The technique used in animal biotechnology for the rapid multiplication and production of animals with a desirable genotype is

Accepted Answer

In vitro fertilization and embryo transfer is a technique used in animal biotechnology for the rapid multiplication and production of animals with desirable genotypes. This process involves fertilizing an egg outside the body and then transferring the embryo into a recipient female.

Question 16

The production of complete animals from somatic cells of an animal is called

Accepted Answer

Animal cloning is the process of producing a genetically identical copy of an animal by using somatic cells from the original animal.

Question 17

In humans, the babies produced by in vitro fertilization and embryo transfer was popularly called as

Accepted Answer

Test tube babies is the popular term used to refer to babies conceived through in vitro fertilization and embryo transfer.

Quiz 2: Biotechnology and Animal Cell Culture