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You Have Transfected SiRNA Against Transcription Factor a from Q

Question 57

Essay

You have transfected siRNA against transcription factor A from Q. 59 into cells that normally express the transcription factor.
(a) What would be the effect on CAT reporter gene expression?
(b) How could you determine whether RNAi acts at the level of mRNA or translation in this case?

Correct Answer:

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(a) The siRNA would knockdown expression...

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