What is the main difference between fluorochrome staining (e.g., auraminerhodamine screening for Mycobacterium tuberculosis) and indirect fluorescent antibody (IFA) staining?

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Fluorochrome dyes, like auramine-rhodamine screening for Mycobacterium tuberculosis, lack the specificity of either direct or indirect FA tests because the FA tests use antibodies, making them more specific. The fluorochrome dyes are more sensitive than a comparable acid-fast stain on a bright field light microscope because they light up the microbes on a black background when viewed with the fluorescent microscope. However, the use of antibodies in the IFAs makes them highly specific.

What Is the Main Difference Between Fluorochrome Staining (E