The ability to incorporate restriction fragments with blunt ends into plasmid vectors linearized with similar enzymes is particularly dependent upon which of the following enzymes?

Question

Quizplus · Accepted Answer

DNA ligase is the enzyme responsible for joining DNA fragments with blunt ends by forming phosphodiester bonds between adjacent nucleotides.  DNA ligase covalently attaches a free 5′-phosphate to a 3′-hydroxyl and it is, therefore, used in all procedures where the molecules of DNA need to be covalently attached.

The Ability to Incorporate Restriction Fragments with Blunt Ends into Plasmid