Question 1

________ are nonviral genetic elements that have reverse transcriptase activity.

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The answer of ________ are nonviral genetic elements that have...

Question 2

You are trying to discover what novel proteins are expressed by a T cell line in response to interaction with a novel antigen associated with HIV.As this is a potential vaccine candidate,you need to see what features of the immune response are initiated by T cells.How would you go about the task?

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The answer of You are trying to discover what novel...

Question 3

________-driven promoters do not contain TATA boxes or BREs.

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The answer of ________-driven promoters do not contain TATA boxes...

Question 4

The ________ activity of RNA polymerase is sufficient for the process of RNA proofreading.

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Question 5

Explain how an electrophoretic mobility shift assay (EMSA)works.How might one use this technique?

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Question 6

You are interested in the novel expression of novel products that are transcribed in response to viral infection by respiratory epithelial cells.How would you go about specifically isolating mRNA in order to begin the process of answering your question? How would this differ if you were using a bacterial model?

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Question 7

The technique initially used in the identification of promoter sequences was ________.

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Question 8

An example of a retrovirus in the human genome is the ________ family of sequences.

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Question 9

________-driven promoters are comprised of an Inr sequence and a TATA box with or without an associated BRE.

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Question 10

A ________ is a complex of RNA and protein that serves to remove introns from pre-mRNA.

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Question 11

In eukaryotic transcription,TFIID recognizes and binds the ________ box in the DNA.

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Question 12

A mutation that results in the addition or deletion of a single nucleotide that radically changes the amino acid sequence of the resulting polypeptide is known as a ________ mutation.

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Question 13

One of the NASA land rovers returns from its mission with a variety of samples.From one,a unique bacterial species has been isolated and cultured.Your job is to investigate whether or not the genetic code is overlapping or nonoverlapping.Another researcher has discovered that rather than a codon of three nucleotides,this organism uses groups of four nucleotides.Show how you would verify the presence of overlapping versus nonoverlapping frames when you thought the codon was read in groups of three nucleotides and in groups of four nucleotides.How would your data have been different without the knowledge of codons as groups of four nucleotides?
Test mRNA sequence: 5'-AUUGGCCAAUUUACGGUAAUGGCCAAUUUACGGU-3'

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Question 14

A more contemporary technique for identification of promoter sequences is the ________ assay.

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Question 15

The coding regions of a gene are known as ________.

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Question 16

You have identified a protein that you feel is a sigma factor in E.coli that has not yet been characterized to date.Design an experiment whereby use can determine that this factor is a sigma factor.

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Question 17

The 21st and 22nd amino acids found in proteins are the rare amino acids ________ and ________.

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Question 18

In order to protect the integrity of an mRNA in the cytosol of a cell,a(n)________ is added to the 3' end during RNA processing.

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Question 19

Match between columns
                        Premises: RNA polymerase
5' mRNA cap
rho (ρ) factor
core RNA polymerase
CAP protein
mRNA
hnRNA
sigma (σ) subunit of RNA polymerase
tRNA
ribozymes

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Quiz 21: Gene Expression: Ithe Genetic Code and Transcription