Question 1

If two different auxotrophic strains are placed in a liquid medium culture tube,prototrophic strains can sometimes be subsequently recovered.Name several mechanisms by which this is possible.

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Question 2

What experimental observation demonstrated that genes are involved in the integration of foreign DNA into the bacterial chromosome?

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Question 3

What is meant by the term cotransformation?

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Question 4

What is a bacteriophage?

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Question 5

What is a merozygote?

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Question 6

What is the consequence of a mutation in the recA gene in bacteria?

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Question 7

Bacteria that are in a particular physiological state to become transformed are called ________.

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Question 8

Explain what is meant by the term heteroduplex in the context of bacterial transformation.

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Question 9

Name the term used to describe the phenomenon in which a bacteriophage genome incorporates its genome into the chromosome of the host.

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Question 10

What is a significant difference between a lytic and a lysogenic cycle?

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Question 11

Name three forms of recombination in bacteria.

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Question 12

A form of bacterial recombination that involves a viral intermediate is called ________.

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Question 13

Bacteriophages that cannot undergo lysogeny but can infect bacteria are called ________.

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Question 14

What are the roles of the recBCD genes?

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Question 15

Compare and contrast bacteriophage lysis and lysogeny.

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Question 16

When a bacteriophage genome incorporates itself into the chromosome of the host,that phage genome is referred to as a(n)________.

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Question 17

Assume that one counted 67 plaques on a bacterial plate where 0.1 ml of a 10⁻⁵ dilution of phage was added to bacterial culture.What is the initial concentration of the undiluted phage?

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Question 18

In a bacterial cross in which the donor (Hfr)is a⁺b⁺ and the recipient strain (F⁻)is a⁻b⁻,it is expected that recombinant bacteria will all be a⁺b⁺.

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In an Hfr-F- cross, the recombinant bacteria will not be a⁺b⁺. While some genes from the donor chromosome may be transferred to the recipient, the entire donor chromosome is not transferred, so the recipient remains a⁻b⁻. Therefore, the resulting recombinants will be a⁺ or b⁺ or both, but not necessarily both together.

Question 19

Present the general structural features of a plasmid and give an example.

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Question 20

Lysogeny is an important phenomenon in bacteria and phages.Briefly describe lysogeny (using labeled diagrams if helpful).

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Quiz 6: Genetic Analysis and Mapping in Bacteria and Bacteriophages