Question 1

Normal incubation temperatures range from 30^$\circ$C to 60^$\circ$C.

Accepted Answer

Normal incubation temperatures range from 86°F (30°C) to 140°F (60°C), not 30°F to 60°F.

Question 2

One colony typically develops from the growth of several parent bacterial cells.

Accepted Answer

A colony typically develops from the growth of a single parent bacterial cell, which divides repeatedly to form a visible mass of cells.

Question 3

The real image is the reverse of the actual specimen.

Accepted Answer

The answer of The real image is the reverse of...

Question 4

The Gram staining procedure is best described as a(n)__ staining technique.

Accepted Answer

Gram staining is a differential staining technique because it differentiates bacteria into two groups based on the properties of their cell walls.

Question 5

Which type of microscope bombards a whole,metal-coated specimen with electrons moving back and forth over it?

Accepted Answer

Scanning electron microscope (SEM) bombards a whole, metal-coated specimen with electrons moving back and forth over it, creating a three-dimensional image of the specimen's surface.

Question 6

Bacterial cultures are easily identified from their microscopic appearance.

Accepted Answer

Bacterial cultures cannot be identified solely based on their microscopic appearance. Other tests such as biochemical tests or genetic analysis may be needed.

Question 7

The specimen preparation that is best for viewing cell motility is

Accepted Answer

A hanging drop preparation suspends the specimen in a drop of liquid on a coverslip, allowing for observation of live, motile cells under a microscope. This technique provides a more natural and dynamic view of cell motility compared to fixed stained smears.

Question 8

All of the following pertain to the fluorescence microscope except it

Accepted Answer

The fluorescence microscope uses light rather than electrons to produce an image.

Question 9

What do the Gram stain,acid-fast stain,and endospore stain have in common?

Accepted Answer

The Gram stain, acid-fast stain, and endospore stain all rely on differences in cell structures to produce a visual outcome. The Gram stain distinguishes between the cell wall composition of Gram-positive and Gram-negative bacteria, the acid-fast stain distinguishes between bacteria with mycolic acid in their cell walls and those without, and the endospore stain distinguishes between cells with endospores and those without.

Question 10

The purpose of staining cells on a microscope slide is to

Accepted Answer

Staining cells on a microscope slide can help to add contrast to the cells, making them easier to see and distinguish from their surroundings. Staining does not necessarily kill or secure the cells, nor does it enlarge them or indicate motility.

Question 11

A microbiologist makes a fixed smear of bacterial cells and stains them with Loeffler's methylene blue.All the cells appear blue under the oil lens.This is an example of

Accepted Answer

When all the bacterial cells appear blue under the oil lens after staining with Loeffler's methylene blue, it indicates that the stain is a simple stain. Simple staining involves the use of a single dye, typically a basic dye, which stains all cells the same color. Negative staining involves using an acidic dye, but in this case, all the cells are stained positively. Acid-fast staining is used to identify bacteria with waxy cell walls, which is not applicable in this scenario. Capsule staining is used to visualize the capsule surrounding some bacterial cells, which is not applicable in this scenario.

Question 12

A confocal scanning microscope

Accepted Answer

Confocal scanning microscopes use laser-based technology to create three-dimensional images of cells from the cell surface to the middle of the cell. They do not rely on stains or electron interaction and can be used with live or fixed specimens.

Question 13

Basic dyes are

Accepted Answer

Basic dyes are positively charged and are attracted to the negatively charged acidic substances of bacterial cells. This attraction allows the dye to attach to the surface of the cell and stain it, making it visible under a microscope. Basic dyes are commonly used for staining bacteria in microbiology.

Question 14

Which type of microscope achieves the greatest resolution and highest magnification?

Accepted Answer

Electron microscopes achieve the greatest resolution and highest magnification compared to light microscopes. They use a beam of electrons to illuminate the specimen, and the resolution is limited only by the wavelength of electrons, which is much shorter than visible light.

Question 15

A selective medium contains one or more substances that inhibit growth of certain microbes in order to facilitate the growth of other microbes.

Accepted Answer

Selective media are designed to suppress the growth of unwanted bacteria, while allowing the growth of desired bacteria. This is achieved through the use of selective agents such as antibiotics or dyes which inhibit the growth of certain microbes.

Question 16

The bending of light rays as they pass from one medium to another is called refraction.

Accepted Answer

This statement is true. Refraction occurs when light passes through a medium with a different refractive index, causing the light rays to bend.

Question 17

Which type of microscope does not use light in forming the specimen image?

Accepted Answer

Electron microscopes use beams of electrons instead of light to form the image of the specimen.

Question 18

Mixed cultures are also referred to as contaminated cultures.

Accepted Answer

Mixed cultures contain multiple types of microorganisms intentionally added for research or industrial purposes, while contaminated cultures have unintentionally introduced microorganisms that can interfere with the intended outcome.

Question 19

The procedures for culturing a microorganism require the use of a microscope.

Accepted Answer

While a microscope may be used to observe and study microorganisms, the actual procedures for culturing them typically involve techniques such as streaking, plating, and incubation in specific growth media.

Question 20

Some microbes are not capable of growing on artificial media.

Accepted Answer

Some microbes have specific nutritional needs or growth requirements that cannot be met by artificial media, leading to their inability to grow on such media.

Quiz 2: Tools of the Laboratory: Methods for the Culturing and Microscopic Analysis

Normal incubation temperatures range from 30^$\circ$C to 60^$\circ$C.

One colony typically develops from the growth of several parent bacterial cells.

The real image is the reverse of the actual specimen.

The Gram staining procedure is best described as a(n) __ staining technique.

Which type of microscope bombards a whole,metal-coated specimen with electrons moving back and forth over it?

Bacterial cultures are easily identified from their microscopic appearance.

The specimen preparation that is best for viewing cell motility is

All of the following pertain to the fluorescence microscope except it

What do the Gram stain,acid-fast stain,and endospore stain have in common?

The purpose of staining cells on a microscope slide is to

A microbiologist makes a fixed smear of bacterial cells and stains them with Loeffler's methylene blue.All the cells appear blue under the oil lens.This is an example of

A confocal scanning microscope

Basic dyes are

Which type of microscope achieves the greatest resolution and highest magnification?

A selective medium contains one or more substances that inhibit growth of certain microbes in order to facilitate the growth of other microbes.

The bending of light rays as they pass from one medium to another is called refraction.

Which type of microscope does not use light in forming the specimen image?

Mixed cultures are also referred to as contaminated cultures.

The procedures for culturing a microorganism require the use of a microscope.

Some microbes are not capable of growing on artificial media.

Quiz 2: Tools of the Laboratory: Methods for the Culturing and Microscopic Analysis

Normal incubation temperatures range from 30 ∘\circ∘ C to 60 ∘\circ∘ C.

One colony typically develops from the growth of several parent bacterial cells.

The real image is the reverse of the actual specimen.

The Gram staining procedure is best described as a(n) __ staining technique.

Which type of microscope bombards a whole,metal-coated specimen with electrons moving back and forth over it?

Bacterial cultures are easily identified from their microscopic appearance.

The specimen preparation that is best for viewing cell motility is

All of the following pertain to the fluorescence microscope except it

What do the Gram stain,acid-fast stain,and endospore stain have in common?

The purpose of staining cells on a microscope slide is to

A microbiologist makes a fixed smear of bacterial cells and stains them with Loeffler's methylene blue.All the cells appear blue under the oil lens.This is an example of

A confocal scanning microscope

Basic dyes are

Which type of microscope achieves the greatest resolution and highest magnification?

A selective medium contains one or more substances that inhibit growth of certain microbes in order to facilitate the growth of other microbes.

The bending of light rays as they pass from one medium to another is called refraction.

Which type of microscope does not use light in forming the specimen image?

Mixed cultures are also referred to as contaminated cultures.

The procedures for culturing a microorganism require the use of a microscope.

Some microbes are not capable of growing on artificial media.

Normal incubation temperatures range from 30^$\circ$C to 60^$\circ$C.