Question 1

__________ can reveal the order of nucleotide bases in a fragment of DNA.

Accepted Answer

The answer of __________ can reveal the order of nucleotide...

Question 2

cDNA libraries are desirable for the expression of eukaryotic genes in prokaryotes because

Accepted Answer

Prokaryotes are unable to remove introns, which are non-coding regions of mRNA that must be removed before translation into protein. cDNA libraries are constructed from mRNA, which has already had introns removed, making it a desirable template for expression in prokaryotes.

Question 3

To answer the following questions refer to the four items listed below.
-This is a collection of host cells that house cloned genomic DNA.

Accepted Answer

A DNA library is a collection of host cells that contain cloned genomic DNA. cDNA is a library of complementary DNA synthesized from mRNA, not genomic DNA. Restriction enzymes and reverse transcriptase are tools used to create a DNA library or cDNA, but they are not the library themselves.

Question 4

To answer the following questions, refer to the five items listed below:
-The combination of a foreign piece of DNA with the DNA of another organism.

Accepted Answer

Recombinant DNA refers to the combination of a foreign piece of DNA with the DNA of another organism, which is one of the items listed.

Answer: A, E
 Restriction enzymes cut DNA at specific sequences known as restriction sites, which are listed as one of the items.

Answer: C
 Plasmids are small, circular pieces of DNA that can be found in bacterial cells and used as vectors to transfer foreign DNA into the cells, which is related to the concept of recombinant DNA listed.

Answer: D
 Clones refer to genetically identical organisms or cells that are produced through asexual reproduction, which is not directly related to any of the items listed.

Question 5

________,a viral enzyme,is used to convert single-stranded RNA into ___________ for cloning.

Accepted Answer

The answer of ________,a viral enzyme,is used to convert single-stranded...

Question 6

To answer the following questions refer to the four items listed below.
-This is a nuclease whose function is to cut apart foreign DNA entering a cell.

Accepted Answer

Restriction enzymes are specialized proteins that can cut DNA molecules at specific locations. They are commonly used in genetic engineering to create recombinant DNA molecules. In nature, some bacteria use restriction enzymes as a defense mechanism against invading viruses by cutting apart the foreign DNA.

Question 7

To answer the following questions refer to the four items listed below.
-This is an enzyme used to produce DNA without introns.

Accepted Answer

Reverse transcriptase is an enzyme that is commonly used to produce cDNA (complementary DNA) from RNA templates. This cDNA lacks introns, which are non-coding regions found in eukaryotic genes. Therefore, choice C is the best choice for the given statement. Restriction enzymes are used in DNA cloning, DNA library construction, and other molecular biology techniques. A DNA library is a collection of DNA fragments that are stored and used for various purposes.

Question 8

To answer the following questions refer to the four items listed below.
-This is any DNA made from mRNA transcripts.

Accepted Answer

cDNA stands for complementary DNA, it is synthesized from single-stranded RNA (mRNA) in a reaction catalyzed by the enzyme reverse transcriptase. This process can be used to create a DNA library containing genes expressed in a particular cell or tissue.

Answer: C 
 Reverse transcriptase is an enzyme that can synthesize a complementary DNA strand from an RNA template. This process is commonly used in molecular biology to create cDNA libraries or to study the expression of genes via reverse transcriptase-PCR (RT-PCR).

Answer: D 
 A DNA library is a collection of DNA fragments derived from a particular organism, tissue, or cell type. These libraries can be used to identify and isolate specific genes of interest, or to study the overall genetic makeup of a given organism or tissue.

B) Restriction enzymes are not related to the production of cDNA or DNA libraries, they are used to cut and manipulate DNA for various molecular biology techniques.

Question 9

In DNA cloning,__________ cut DNA into pieces.

Accepted Answer

The answer of In DNA cloning,__________ cut DNA into pieces....

Question 10

Studying the genomes of other species is helpful for the following except

Accepted Answer

Studying the genomes of other species is primarily focused on understanding genetic functions, evolutionary history, and inheritance patterns, rather than shifts in protein production within an organism, which is more related to proteomics than genomics.

Question 11

What is the main goal for making engineered "golden rice"?

Accepted Answer

The main goal for making engineered "golden rice" is to make rice more nutritious by adding beta-carotene, which is the precursor of Vitamin A.

Question 12

To answer the following questions refer to the four items listed below.
-This is from a viral source and catalyzes reactions to construct DNA strands from mRNA.

Accepted Answer

Reverse transcriptase is an enzyme that can synthesize DNA from an RNA template. This process is known as reverse transcription and is often used in the laboratory to make a complementary DNA (cDNA) copy of an mRNA molecule. This cDNA can then be amplified and cloned to create a DNA library. Restriction enzymes, on the other hand, are used to cut DNA at specific sequences, and are not directly involved in the construction of DNA strands from mRNA.

Question 13

The polymerase chain reaction (PCR)uses ________,nucleotides,and a heat-resistant DNA polymerase to rapidly increase the number of molecules of a DNA fragment.

Accepted Answer

The answer of The polymerase chain reaction (PCR)uses ________,nucleotides,and a...

Question 14

To answer the following questions, refer to the five items listed below:
-These are bacterial populations containing thousands or millions of identical copies of one to several genes.

Accepted Answer

The description of bacterial populations containing identical copies of one to several genes refers to clones, which are commonly used in genetic engineering and biotechnology.

Answer: C
 Plasmids, circular DNA molecules found in bacteria, are commonly used as vectors to carry foreign DNA, such as recombinant DNA or cloned genes, into bacterial cells.

Answer: A and E
 Restriction enzymes recognize and cut specific DNA sequences at restriction sites, which can be used to create DNA fragments that can be manipulated in genetic engineering and biotechnology.

Question 15

To answer the following questions, refer to the five items listed below:
-The pieces of DNA that are moved by a genetic engineer from one organism to another are first incorporated into these.

Accepted Answer

Plasmids are small, circular pieces of DNA that are commonly used as a vector to transfer genetic material from one organism to another during genetic engineering. The foreign DNA is incorporated into the plasmid, which can then be transferred into the target organism through various methods. Restriction enzymes recognize and cut specific sequences of DNA, while restriction sites are the locations where these enzymes cut. Recombinant DNA refers to DNA that has been artificially created by combining genetic material from different sources. Clones are genetically identical copies of an organism or a piece of DNA. While all of these terms are related to genetic engineering, plasmids are the specific structures that are used to transfer genetic material in this context.

Question 16

A process in which a gene fragment is delivered to an individual's target chromosome and spread throughout a population,such as that used to prevent transmission of malaria through mosquitoes,is called a _________.

Accepted Answer

A gene drive is a process that allows a gene fragment to spread rapidly through a population. It has been proposed as a way to prevent transmission of diseases such as malaria by introducing genes into mosquito populations that make them resistant to the disease. Gene translocation refers to the movement of genetic material between chromosomes, gene fragmentation refers to the breaking of a single gene into smaller fragments, and gene library refers to a collection of cloned DNA fragments that are used for research purposes. Gene disk is not a known term in genetics.

Question 17

To answer the following questions, refer to the five items listed below:
-The function of these is to cut apart foreign DNA molecules.

Accepted Answer

Restriction enzymes are specifically designed to cut foreign DNA molecules at specific restriction sites, which are usually palindromic sequences. This is an important tool in genetic engineering, as it allows the manipulation of DNA sequences by cutting and splicing them in different configurations, leading to the creation of recombinant DNA molecules and the generation of cloned DNA fragments. Plasmids are small circular pieces of DNA that can be used to carry foreign DNA sequences into a host cell, and are often used in recombinant DNA technology. Clones are genetically identical copies of a single cell or organism, and are often generated by the replication of DNA fragments that have been inserted into host cells using recombinant DNA techniques.

Question 18

__________ are multiple copies of a short DNA sequence that occur at a given locus.

Accepted Answer

The answer of __________ are multiple copies of a short...

Quiz 15: Studying and Manipulating Genomes

__________ can reveal the order of nucleotide bases in a fragment of DNA.

cDNA libraries are desirable for the expression of eukaryotic genes in prokaryotes because

To answer the following questions refer to the four items listed below. -This is a collection of host cells that house cloned genomic DNA.

To answer the following questions, refer to the five items listed below: -The combination of a foreign piece of DNA with the DNA of another organism.

,a viral enzyme,is used to convert single-stranded RNA into ___ for cloning.

To answer the following questions refer to the four items listed below. -This is a nuclease whose function is to cut apart foreign DNA entering a cell.

To answer the following questions refer to the four items listed below. -This is an enzyme used to produce DNA without introns.

To answer the following questions refer to the four items listed below. -This is any DNA made from mRNA transcripts.

In DNA cloning,__________ cut DNA into pieces.

Studying the genomes of other species is helpful for the following except

What is the main goal for making engineered "golden rice"?

To answer the following questions refer to the four items listed below. -This is from a viral source and catalyzes reactions to construct DNA strands from mRNA.

The polymerase chain reaction (PCR)uses ________,nucleotides,and a heat-resistant DNA polymerase to rapidly increase the number of molecules of a DNA fragment.

To answer the following questions, refer to the five items listed below: -These are bacterial populations containing thousands or millions of identical copies of one to several genes.

To answer the following questions, refer to the five items listed below: -The pieces of DNA that are moved by a genetic engineer from one organism to another are first incorporated into these.

A process in which a gene fragment is delivered to an individual's target chromosome and spread throughout a population,such as that used to prevent transmission of malaria through mosquitoes,is called a _________.

To answer the following questions, refer to the five items listed below: -The function of these is to cut apart foreign DNA molecules.

__________ are multiple copies of a short DNA sequence that occur at a given locus.