How many enzymes should be used to cut the plasmid DNA and the insert DNA if there is a desire to ligate the insert within the vector in a specific orientation?

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Two enzymes should be used to cut the plasmid DNA and the insert DNA if there is a desire to ligate the insert within the vector in a specific orientation. One enzyme should cut the plasmid DNA at the desired site, and the other enzyme should cut the insert DNA at a complementary site to ensure the insert is correctly and specifically oriented when ligated into the plasmid.

How Many Enzymes Should Be Used to Cut the Plasmid