In constructing a cDNA library,which of the following would you use to generate rare restriction sites on the cDNA strands?

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Ligation of specific linkers to cDNA strands creates rare restriction sites that can be used to aid in the construction of a cDNA library. This allows for easier identification and isolation of specific cDNA fragments from the library. Restriction enzymes, PCR with multiple cycles, and BamHI restriction followed by ligation may create more common restriction sites and result in a library with less diversity. 5' and 3' RACE are not relevant to this question.

In Constructing a CDNA Library,which of the Following Would You