If the universal adapter sequence is GTCATTGCTTGCAATGTT,which universal primer should be used for DNA sequencing?
A) GTCATTGCTTGCAATGTT
B) TTGTAACGTTCGTTACTG
C) CAGTAACGAACGTTACAA
D) CCTGAATTGGTCGTACAA
E) GUCAUUGCUUGCAAUGUU

Question

Quizplus · Accepted Answer

To perform DNA sequencing using the Sanger method, we need a primer that anneals to the template DNA and initiates DNA synthesis. The universal adapter sequence given above does not have a complementary sequence that can be used as a primer. However, we can design a universal primer that has a complementary sequence to the adapter sequence. This primer should start from a known region, such as the vector or the plasmid sequence, and extend into the inserted DNA fragment. 
Based on the given adapter sequence, the complementary sequence is 5'-AACGTTACAGTAA-3', and a suitable primer would be 5'-CAGTAACGAACGTTACAA-3' (Choice C). This primer has a Tm value of around 58-60°C and is long enough to provide good specificity and coverage of the DNA fragment during sequencing. The other choices are either too short, too dissimilar to the adapter sequence, or not complementary to the DNA fragment of interest.

If the Universal Adapter Sequence Is GTCATTGCTTGCAATGTT,which Universal Primer Should