What is one major limitation of two-dimensional gel electrophoresis (2-DGE)?
A) It is extremely costly to execute in a typical molecular biology laboratory.
B) When products are separated, they tend to leach out of the gel matrix.
C) Only the most abundant products are detected.
D) 2-DGE can be run only on nucleic acids.
E) 2-DGE is useful only in separating eukaryotic gene products.

Question

Quizplus · Accepted Answer

Two-dimensional gel electrophoresis (2-DGE) has a limitation in which it only detects the most abundant products. This means that lower abundance proteins are often missed, leading to an incomplete understanding of the sample being analyzed.

What Is One Major Limitation of Two-Dimensional Gel Electrophoresis (2-DGE)