Which of the following statements about the polymerase chain reaction (PCR)is false?
A)DNA amplified by PCR can be cloned.
B)DNA amplification is linear in magnitude.
C)Newly synthesized DNA must be heat-denatured before the next round of DNA synthesis begins.
D)The boundaries of the amplified DNA segment are determined by the synthetic oligonucleotides used to prime DNA synthesis.
E)The technique is sufficiently sensitive that DNA sequences can be amplified from a single animal or human hair.

Question

Quizplus · Accepted Answer

The statement that DNA amplification in PCR is linear in magnitude is false. In fact, it is an exponential process where the number of DNA copies produced doubles with each cycle of PCR.

Which of the Following Statements About the Polymerase Chain Reaction