Which of the following is not needed in 454 pyrosequencing of DNA?
A)dNTPs
B)Sulfurylase
C)Luciferase
D)ddNTPs
E)Apyrase

Name: Which of the following is not needed in 454 pyrosequencing of DNA? A)dNTPs B)Sulfurylase C)Luciferase D)ddNTPs E)Apyrase
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Question

Quizplus · Accepted Answer

ddNTPs are not used in 454 pyrosequencing, as they are used in Sanger sequencing but not in pyrosequencing. The other components listed (dNTPs, sulfurylase, luciferase, and apyrase) are all necessary for the pyrosequencing reaction.

Which of the Following Is Not Needed in 454 Pyrosequencing