When double-stranded DNA is heated at neutral pH,which change does not occur?
A)The absorption of ultraviolet (260 nm)light increases.
B)The covalent N-glycosidic bond between the base and the pentose breaks.
C)The helical structure unwinds.
D)The hydrogen bonds between A and T break.
E)The viscosity of the solution decreases.

Question

Quizplus · Accepted Answer

Heating double-stranded DNA at neutral pH can cause the helical structure to unwind (C) and the hydrogen bonds between A and T (D) or G and C to break, resulting in denaturation of the DNA. The absorption of ultraviolet light (A) also increases due to the separation of the nitrogenous bases. The viscosity of the solution (E) decreases due to the loss of the double helix structure. However, the covalent N-glycosidic bond between the base and the pentose (B) does not break during the denaturation process.

When Double-Stranded DNA Is Heated at Neutral PH,which Change Does