In a plot of l/V against 1/[S] for an enzyme-catalyzed reaction, the presence of a competitive inhibitor will alter the: A) curvature of the plot. B) intercept on the l/[S] axis. C) intercept on the l/V axis. D) pK of the plot. E) V_max.

Question

Quizplus · Accepted Answer

A competitive inhibitor decreases the effective concentration of the enzyme by binding to the active site, competing with the substrate. This results in an increase in the apparent Km value for the substrate. As a result, the intercept on the l/[S] axis increases in the presence of a competitive inhibitor. The curvature of the plot remains the same, and the intercept on the l/V axis and pK of the plot are not affected by the presence of a competitive inhibitor. V_max is not a relevant parameter in this context.

In a Plot of L/V Against 1/[S] for an Enzyme-Catalyzed