What is the advantage of using an indirect sandwich assay for EIA?

Question

Quizplus · Accepted Answer

In an indirect sandwich EIA, a primary antibody specific to the antigen of interest is first coated onto the well. Then, a second antibody conjugated to an enzyme is added, which recognizes and binds to the primary antibody. This method has the advantage that the same conjugated antibody can be used for multiple assays, saving on cost and increasing efficiency. However, it may not necessarily be more specific or sensitive than direct sandwich EIA, and the lower detection limit depends on the specific assay and conditions used.

What Is the Advantage of Using an Indirect Sandwich Assay