What is the difference between dot blots and Western blots? In dot blot testing

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Quizplus · Accepted Answer

In dot blot testing, proteins are purified and directly blotted to specific locations on the solid surface. In Western blotting, proteins are separated by size through gel electrophoresis before being transferred to a solid surface and probed with antibodies. Therefore, the key difference between dot blots and Western blots lies in the method of protein separation before blotting.

What Is the Difference Between Dot Blots and Western Blots