When performing a Gram stain on a gram-negative organism, the crystal violet is absorbed into this outer cell wall layer, and then washed away with the acetone alcohol. What is the main component of the outer layer of the cell wall?

Question

Quizplus · Accepted Answer

Lipopolysaccharide is the main component of the outer layer of the cell wall in gram-negative organisms. This component is responsible for the negative charge and endotoxin activity associated with these bacteria. Peptidoglycan is found in both gram-positive and gram-negative bacteria, while mycolic acid and N-acetyl-D-muramic acid are specific to certain bacterial groups (e.g. mycobacteria and gram-positive bacteria, respectively).

When Performing a Gram Stain on a Gram-Negative Organism, the Crystal