The Gram stain is a routine stain used in bacteriology to determine gram-positive and gram-negative bacteria based on the

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The Gram stain is based on the composition of the bacterial cell wall. Gram-positive bacteria have a thick peptidoglycan layer in their cell wall that retains the crystal violet stain used in the Gram stain, while Gram-negative bacteria have a thin peptidoglycan layer and an outer membrane that prevents the crystal violet stain from attaching, but allows the counterstain safranin to be retained. This distinction is important in identifying bacteria and determining appropriate treatments.

The Gram Stain Is a Routine Stain Used in Bacteriology