Immunophenotyping is performed on a patient suspected of having PNH. What monoclonal antibody specificity should be ordered?
A) CD33 and CD13
B) CD56 and CD4
C) CD3 and CD8
D) CD55 and CD59

Question

Quizplus · Accepted Answer

CD55 and CD59 are the monoclonal antibodies used for immunophenotyping in a patient suspected of having PNH because PNH is characterized by the absence or reduced expression of these complement regulatory proteins on the surface of red blood cells, leading to complement-mediated hemolysis. CD33 and CD13 are markers for myeloid cells, CD56 is a marker for natural killer cells, CD4 and CD8 are markers for T cells. Therefore, they are not relevant to PNH diagnosis.

Immunophenotyping Is Performed on a Patient Suspected of Having PNH