Both dideoxy terminator and next-generation sequencing methods require a genomic library to be constructed prior to sequencing the DNA of interest.

Question

Quizplus · Accepted Answer

Next-generation sequencing methods do not necessarily require a genomic library to be constructed in the traditional sense of cloning DNA fragments into vectors. Instead, they often involve direct fragmentation of DNA, followed by adapter ligation and sequencing, bypassing the need for cloning into vectors for library preparation.

Both Dideoxy Terminator and Next-Generation Sequencing Methods Require a Genomic