You want to insert your favorite gene,which has been digested with Ncol endonuclease on both ends,into a vector.Ncol recognizes the DNA sequence C*CATGG.The vector you want to use contains many endonuclease recognition sites,but does not include an Ncol site.Which restriction endonuclease would be the most suitable replacement to digest the vector?
A)Sphl - G*CATGC
B)Sunl - C*GTACG
C)Kpnl - GGTAC*C
D)CViAll - C*ATG

Question

Quizplus · Accepted Answer

Sphl has a DNA sequence recognition of GCATGC, which does not contain the Ncol cut site. Therefore, it can be used to digest the vector without affecting the Ncol digested gene insert.

You Want to Insert Your Favorite Gene,which Has Been Digested