Mutations That Result in a Single Amino Acid Substitution in an Enzyme

Mutations that result in a single amino acid substitution in an enzyme

A) cannot affect the activity or properties of the enzyme.
B) will destroy the activity of the enzyme.
C) may, in rare cases, cause the enzyme to run reactions in reverse.
D) will change the substrate specificity of the enzyme.
E) may change the enzyme's optimal temperature or optimal pH.