How Might a Change of One Amino Acid at a Site

How might a change of one amino acid at a site, distant from the active site of an enzyme, alter an enzyme's substrate specificity?

A) by changing the enzyme's pH optimum
B) by changing the enzyme's binding of allosteric regulator
C) by changing the enzyme's location in the cell
D) by changing the shape of an enzyme
E) An amino acid change away from the active site cannot alter the enzyme's substrate specificity.